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Caspase 3 Apoptosis Extract(PDF)


Product Number Amount/Size Price
#E0003 10 Western Blots $90/set


Description

Total cell lysates collected from Jurkat cells (human lymphoma T cell line) were treated with vehicle (-AD) or Actinomycin D (+AD) and lysed in CHAPS Cell extract buffer (B0001). This treatment induces the apoptotic activation of caspase 3 to the cleaved and active form and serve as molecular weight controls for Western blot analysis.


Conditions

Provided as a lyophilized sample. Resuspend each of the (+) and (-) Caspase 3 control extracts in 100ul of 1X Laemmli Reducing Sample Buffer. Distribute and label each of the (+) and (-) samples into 10ul aliquots and store at -20C.


Applications

10ul/well Western Blot; Load 10ul of positive and 10ul of negative control and lysates per lane. To use: heat a 10ul of positive and 10ul of negative aliquot at 95C for 3 minutes and load on a Std SDS-PAGE gel.


Background

Caspase 3 Apoptosis Extract Caspase-3 is a critical effector caspase of the apoptotic pathway, which is activated after being cleaved by initiator caspases (1,2). Caspase-3 is activated by cleaving the 35kDa inactive zymogen into 17/19kDa active units.


Storage Conditions

Store at -20ÂșC. Avoid repeated freeze/thaw cycles.


References

1. Caserta TM, Smith AN, Gultice AD, Reedy MA, and Brown TL. 2003. Q-VD-OPh, a broad spectrum caspase inhibitor with potent antiapoptotic properties. Apoptosis 8: 345-352
2. Renolleau S, Fau S, Goyenvalle C, Joly LM, Chauvier D, Jacotot E, Mariani J, Charriaut-Marlangue C. Specific caspase inhibitor Q-VD-OPh prevents neonatal stroke in P7 rat: a role for gender. J Neurochem. 2007 100:1062-71.
3. Southerland B, Kulkarni-Datar K, Keoni C, Bricker R, Grunwald WC, Ketcha DM, Hern E, Cool DR and Brown TL. Q-VE-OPh, a negative control for O-phenoxy-conjugated caspase inhibitors. 2010 Journal of Cell Death 3:33-40


Companion Products

Q-VE-OPh Apoptosis Negative Control (#A0007)
Caspase 9 Apoptosis Extracts (#E0009)
Caspase 3 Apoptosis Extracts (#E0003)
Caspase 8 Apoptosis Extracts (#E0008)
XIAP Apoptosis Extracts (#S0002)
BID Apoptosis Extracts (#S0003)
CHAPS Cell Extract Buffer (#B0001)


For research purposes only. Not for use in humans.

Apoptrol, LLC
PO Box 32, Enon, OH 45323-0032
Phone: 937-623-8729