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Caspase 9 Apoptosis Extract(PDF)


Product Number Amount/Size Price
#E0009 10 Western Blots $90/set


Description

Total cell lysates isolated from Jurkat human T cells were treated with vehicle (-) or Actinomycin D (+AD) and lysed in CHAPS Cell extract buffer (#B0001). These treatments induce the apoptotic activation of procaspase-9 to the cleaved and active form of caspase 9 and serve as molecular weight controls for Western blot analysis.


Conditions

Provided as a lyophilized sample. Resuspend each of the (+) and (-) caspase 9 control extracts in 100ul of 1X Laemmli Reducing Sample Buffer. Distribute and label each of the (+) and (-) samples into 10ul aliquots and store at -20C.


Applications

10ul/well Western Blot; Load 10ul of positive and 10ul of negative control and lysates per lane. To use: heat a 10ul of positive and 10ul of negative aliquot at 95C for 3 minutes and load on a Std SDS-PAGE gel.


Background

Caspase 9 Apoptosis Extract Caspase-9 (MCH6, APAF3, ICE-LAP6) is a critical initiator caspase of the apoptotic pathway, which is activated after the triggered mitochondria releases cytochrome c. Caspase-9 is activated by cleaving the ~47kDa inactive zymogen into 35kDa and 17kDa active subunits and occurs following aspartate residues (1-3).


References

1. Caserta TM, Smith AN, Gultice AD, Reedy MA, and Brown TL. 2003. Q-VD-OPh, a broad spectrum caspase inhibitor with potent antiapoptotic properties. Apoptosis 8, 345-352
2. Zou H, Li Y, Liu X, and Wang X. An APAF-1•Cytochrome c Multimeric Complex Is a Functional Apoptosome That Activates Procaspase-9 .1999. J Biol Chem 274, 11549-11556
3. Southerland B, Kulkarni-Datar K, Keoni C, Bricker R, Grunwald WC, Ketcha DM, Hern E, Cool DR and Brown TL. Q-VE-OPh, a negative control for O-phenoxy-conjugated caspase inhibitors. 2010 Journal of Cell Death 3:33-40


Companion Products

Q-VE-OPh Apoptosis Negative Control (#A0007)
Caspase 3 Apoptosis Extracts (#E0003)
Caspase 8 Apoptosis Extracts (#E0008)
PARP Apoptosis Extracts (#S0001)
XIAP Apoptosis Extracts (#S0002)
BID Apoptosis Extracts (#S0003)
CHAPS Cell Extract Buffer (#B0001)


For research purposes only. Not for use in humans.

Apoptrol, LLC
PO Box 32, Enon, OH 45323-0032
Phone: 937-623-8729